Volume 8 Issue 1
Jun.  2024
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Wei Geng, Fansheng Kong, Huanhuan Gao, Hongjin Chang, Jiahui Wang, Na Zhang, Dongqing Han, Yan Zhao. The HLA Epitope Donor Database for Platelet Matching[J]. Blood&Genomics, 2024, 8(1): 10003. DOI: 10.70322/BG20240110003
Citation: Wei Geng, Fansheng Kong, Huanhuan Gao, Hongjin Chang, Jiahui Wang, Na Zhang, Dongqing Han, Yan Zhao. The HLA Epitope Donor Database for Platelet Matching[J]. Blood&Genomics, 2024, 8(1): 10003. DOI: 10.70322/BG20240110003
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The HLA Epitope Donor Database for Platelet Matching

  • 1 Blood Grouping Laboratory, Jining Blood Center, Jining 272041, China;

  • 2 Department of Blood Transfusion, the Affiliated Hospital of Jining Medical University, Jining 272029, China

Funds: 

This work was supported by the Projects of Medical and Health Technology Development Program in Shandong (No. 202102070720).

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  • Received Date: 2024-03-11
  • Accepted Date: 2024-06-29
  • Available Online: 2024-06-27
  • Published Date: 2025-06-21
    Graphical Abstract
    • Abstract
  • This study aims to create a platelet donor database with detailed HLA epitope typing in the Jining area and to serve as a solid foundation for treating refractory thrombocytopenia patients who require platelet transfusions. HLA-A, -B epitope phenotype data were collected from a total of 565 unrelated healthy populations in the Jining area. According to the data, a platelet donor database was created and established with known HLA epitope phenotypes. The database donors' HLA-A, -B epitopes were tested separately by sequence-specific primer (PCR-SSP). According to the distribution frequency of HLA-A, -B epitope phenotypes (n = 565), the following results were obtained: 73 HLA-A, -B phenotypes, including 55 with a frequency > 0.003. The sum of frequencies was 96.81%. With a database size of 565 donors, an approximate 84.20% patient HLA-A, -B cross-reactive group (CREG) match coverage was achieved. When it was necessary for identical ABO-type platelet transfusion, the corresponding database modifications were made according to the distribution frequency of the ABO blood group of the population.This study provides the concept for a platelet epitope matching database. It establishes a donor database of known platelet HLA epitopes in the Jining population, which lays a solid foundation for the safe treatment of platelet transfusion in this area.
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    • References (24)
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