Volume 5 Issue 2
Dec.  2021
Turn off MathJax
Article Contents
Article Navigation >  Blood&Genomics  > 2021  >  5(2): 128-134
Fuhua Wang, Yunfeng Yang, Xiangyun Guo, Feng Li, Ruihong Yang, Xiaoyu Li, Sutang Guo, Chunyan Wang. Plasma microRNA profiling highlights miR-1260b and miR-720 as novel diagnostic and prognostic biomarkers of esophageal squamous cell carcinoma[J]. Blood&Genomics, 2021, 5(2): 128-134. doi: 10.46701/BG.2021022021115
Citation: Fuhua Wang, Yunfeng Yang, Xiangyun Guo, Feng Li, Ruihong Yang, Xiaoyu Li, Sutang Guo, Chunyan Wang. Plasma microRNA profiling highlights miR-1260b and miR-720 as novel diagnostic and prognostic biomarkers of esophageal squamous cell carcinoma[J]. Blood&Genomics, 2021, 5(2): 128-134. doi: 10.46701/BG.2021022021115
shu

Plasma microRNA profiling highlights miR-1260b and miR-720 as novel diagnostic and prognostic biomarkers of esophageal squamous cell carcinoma

doi: 10.46701/BG.2021022021115
  • 1.

    Department of Molecular Biology, Shanxi Cancer Hospital and Institute, Taiyuan, Shanxi 030013, China

  • 2.

    Department of Gastroenterology, Shanxi Provincial People's Hospital, Taiyuan, Shanxi 030012, China

  • 3.

    Department of Blood Transfusion, Shanxi Cancer Hospital and Institute, Taiyuan, Shanxi 030013, China

More Information
  • Corresponding author: Sutang Guo, Department of Molecular Biology, Shanxi Cancer Hospital and Institute, Taiyuan, Shanxi 030013, China. E-mail: sutangguo@aliyun.com; Chunyan Wang, Department of Molecular Biology, Department of Blood Transfusion, Shanxi Cancer Hospital and Institute, Taiyuan, Shanxi 030013, China. E-mail: c3213258@uon.edu.au
  • Received Date: 2021-04-18
  • Accepted Date: 2021-09-01
  • Rev Recd Date: 2021-06-15
    • Available Online: 2022-01-06
  • Publish Date: 2021-12-31
    Abstract
  • This paper was designed to explore the value of miRNAs as diagnostic biomarkers that may facilitate the early detection of esophageal squamous cell carcinoma (ESCC). Plasma miRNA profiles were defined via an array-based approach using samples from ESCC patients and healthy controls (n=5 each). Differentially expressed miRNAs in these samples were validated via qPCR in ESCC patients (n=96) and healthy controls (n=51), and the relationship between ESCC patient plasma miR-1260b and miR-720 levels and clinicopathological characteristics were additionally examined. In total, 12 plasma miRNAs that were differentially expressed between ESCC patients and healthy controls were identified via miRNA. Six of these miRNAs were subsequently validated, revealing that both miR-1260b and miR-720 were significantly differentially abundant in ESCC patients and controls, with miR-1260b being significantly upregulated in ESCC patients relative to controls (2.24, 1.41 respectively, P<0.001), while the opposite was observed with respect to miR-720 (0.66, 2.27 respectively, P=0.001). The use of both miR-720 and miR-1260b as a combined diagnostic tool was highly efficacious, yielding an AUC of 0.814, a sensitivity of 86.3%, and a specificity of 73.2% as a means of detecting ESCC patients. Elevated plasma miR-1260b level was also associated with a poorer patient prognosis when compared to patients with a low plasma miRNA level (P=0.021). This study has successfully developed a plasma miRNA biomarker signature of ESCC that may offer value as a diagnostic or prognostic tool when evaluating patients with ESCC.

     

    • △These authors contributed equally to this work
    FullText(HTML)
  • loading
    • References(30)
  • [1]
    Bray F, Ferlay J, Soerjomataram I, et al. Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries[J]. CA Cancer J Clin, 2018, 68(6): 394−424. doi: 10.3322/caac.21492
    [2]
    Abnet CC, Arnold M, Wei WQ. Epidemiology of esophageal squamous cell carcinoma[J]. Gastroenterology, 2018, 154(2): 360−373. doi: 10.1053/j.gastro.2017.08.023
    [3]
    Malhotra GK, Yanala U, Ravipati A, et al. Global trends in esophageal cancer[J]. J Surg Oncol, 2017, 115(5): 564−579. doi: 10.1002/jso.24592
    [4]
    Wu CC, Li M, Meng HB, et al. Analysis of status and countermeasures of cancer incidence and mortality in China[J]. Sci China Life Sci, 2019, 62(5): 640−647. doi: 10.1007/s11427-018-9461-5
    [5]
    Cao MM, Li H, Sun DQ, et al. Cancer burden of major cancers in China: a need for sustainable actions[J]. Cancer Commun, 2020, 40(5): 205−210. doi: 10.1002/cac2.12025
    [6]
    He YT, Li DJ, Liang D, et al. Estimated of esophageal cancer incidence and mortality in China, 2013[J]. Chin J Oncol (in Chinese), 2017, 39(4): 315−320. doi: 10.3760/cma.j.issn.0253-3766.2017.04.016
    [7]
    Liu S, Lin Z, Zheng ZR, et al. Serum exosomal microRNA-766-3p expression is associated with poor prognosis of esophageal squamous cell carcinoma[J]. Cancer Sci, 2020, 111(10): 3881−3892. doi: 10.1111/cas.14550
    [8]
    Kawaguchi T, Komatsu S, Ichikawa D, et al. Circulating MicroRNAs: a next-generation clinical biomarker for digestive system cancers[J]. Int J Mol Sci, 2016, 17(9): 1459. doi: 10.3390/ijms17091459
    [9]
    Wang H, Peng R, Wang J, et al. Circulating microRNAs as potential cancer biomarkers: the advantage and disadvantage[J]. Clin Epigenetics, 2018, 10: 59. doi: 10.1186/s13148-018-0492-1
    [10]
    Carthew RW, Sontheimer EJ. Origins and mechanisms of miRNAs and siRNAs[J]. Cell, 2009, 136(4): 642−655. doi: 10.1016/j.cell.2009.01.035
    [11]
    Ali Syeda Z, Langden SSS, Munkhzul C, et al. Regulatory mechanism of microRNA expression in cancer[J]. Int J Mol Sci, 2020, 21(5): 1723. doi: 10.3390/ijms21051723
    [12]
    Lu J, Getz G, Miska EA, et al. MicroRNA expression profiles classify human cancers[J]. Nature, 2005, 435(7043): 834−838. doi: 10.1038/nature03702
    [13]
    Hoshino I, Ishige F, Iwatate Y. Usefulness of serum miR-1246/miR-106b ratio in patients with esophageal squamous cell carcinoma[J]. Oncol Lett, 2020, 20(6): 350. doi: 10.3892/ol.2020.12213
    [14]
    Liu C, Tian X, Sun HB, et al. MiR-601 inhibits the proliferation and metastasis of esophageal squamous cell carcinoma (ESCC) by targeting HDAC6[J]. Eur Rev Med Pharmacol Sci, 2019, 23(3): 1069−1076. doi: 10.26355/eurrev_201902_16995
    [15]
    Shen Y, Ding YJ, Ma Q, et al. Identification of novel circulating miRNA biomarkers for the diagnosis of esophageal squamous cell carcinoma and squamous dysplasia[J]. Cancer Epidemiol Biomarkers Prev, 2019, 28(7): 1212−1220. doi: 10.1158/1055-9965.EPI-18-1199
    [16]
    Sun HJ, Wang L, Zhao QQ, et al. Diagnostic and prognostic value of serum miRNA-1290 in human esophageal squamous cell carcinoma[J]. Cancer Biomark, 2019, 25(4): 381−387. doi: 10.3233/CBM-190007
    [17]
    Wang LH, Zhang ZY, Yu XD, et al. SOX9/miR-203a axis drives PI3K/AKT signaling to promote esophageal cancer progression[J]. Cancer Lett, 2020, 468: 14−26. doi: 10.1016/j.canlet.2019.10.004
    [18]
    Li ML, Meng XL, Li MX. MiR-126 promotes esophageal squamous cell carcinoma via inhibition of apoptosis and autophagy[J]. Aging, 2020, 12(12): 12107−12118. doi: 10.18632/aging.103379
    [19]
    Lu TX, Rothenberg ME. MicroRNA[J]. J Allergy Clin Immunol, 2018, 141(4): 1202−1207. doi: 10.1016/j.jaci.2017.08.034
    [20]
    Kabekkodu SP, Shukla V, Varghese VK, et al. Clustered miRNAs and their role in biological functions and diseases[J]. Biol Rev Camb Philos Soc, 2018, 93(4): 1955−1986. doi: 10.1111/brv.12428
    [21]
    Backes C, Meese E, Keller A. Specific miRNA disease biomarkers in blood, serum and plasma: challenges and prospects[J]. Mol Diagn Ther, 2016, 20(6): 509−518. doi: 10.1007/s40291-016-0221-4
    [22]
    Hirata H, Hinoda Y, Shahryari V, Deng G, Tanaka Y, Tabatabai ZL et al. Genistein downregulates onco-miR-1260b and upregulates sFRP1 and Smad4 via demethylation and histone modification in prostate cancer cells[J]. Br J Cancer, 2014, 110(6): 1645−1654. doi: 10.1038/bjc.2014.48
    [23]
    Xia Y, Wei K, Hu LQ, et al. Exosome-mediated transfer of miR-1260b promotes cell invasion through Wnt/β-catenin signaling pathway in lung adenocarcinoma[J]. J Cell Physiol, 2020, 235(10): 6843−6853. doi: 10.1002/jcp.29578
    [24]
    Morita T, Fujiwara T, Yoshida A, et al. Clinical relevance and functional significance of cell-free microRNA-1260b expression profiles in infiltrative myxofibrosarcoma[J]. Sci Rep, 2020, 10(1): 9414. doi: 10.1038/s41598-020-66120-8
    [25]
    Xu LM, Xu XT, Huang HL, et al. MiR-1260b promotes the migration and invasion in non-small cell lung cancer via targeting PTPRK[J]. Pathol Res Pract, 2018, 214(5): 776−783. doi: 10.1016/j.prp.2018.02.002
    [26]
    Nonaka R, Miyake Y, Hata T, et al. Circulating miR-103 and miR-720 as novel serum biomarkers for patients with colorectal cancer[J]. Int J Oncol, 2015, 47(3): 1097−1102. doi: 10.3892/ijo.2015.3064
    [27]
    Tang Y, Lin Y, Li C, et al. MicroRNA-720 promotes in vitro cell migration by targeting Rab35 expression in cervical cancer cells[J]. Cell Biosci, 2015, 5: 56. doi: 10.1186/s13578-015-0047-5
    [28]
    Chen P, Zhang GY, Zhou Q, et al. Plasma microRNA-720 may predict prognosis and diagnosis in glioma patients[J]. Biosci Rep, 2020, 40(7): BSR20201449. doi: 10.1042/BSR20201449
    [29]
    Lv ZY, Wang S, Zhao WD, et al. MicroRNA analysis of NCI-60 human cancer cells indicates that miR-720 and miR-887 are potential therapeutic biomarkers for breast cancer[J]. Drug Discov Ther, 2020, 14(4): 197−203. doi: 10.5582/ddt.2020.03058
    [30]
    Shinozuka E, Miyashita M, Mizuguchi Y, et al. SnoN/SKIL modulates proliferation through control of hsa-miR-720 transcription in esophageal cancer cells[J]. Biochem Biophys Res Commun, 2013, 430(1): 101−106. doi: 10.1016/j.bbrc.2012.11.005
    • Relative Articles
    • Supplements(0)
    • Cited By
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Figures(5)  / Tables(1)

    Get Citation
    PDF
    XML

    Article Metrics

    Article views (200) PDF downloads(10) Cited by()
    Proportional views
    Related

    Copyright © Blood and Genomics苏公网安备 32028102000501号苏ICP备14047883号-6

    Address：Rooms 1318-19, Hollywood Plaza, 610 Nathan Road, Mongkok, Kowloon, HongKong

    E-mail：editorial_office@apjbg.com

    Supported by: Beijing Renhe Information Technology Co. Ltd    

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return