The HLA Epitope Donor Database for Platelet Matching

This study aims to create a platelet donor database with detailed HLA epitope typing in the Jining area and to serve as a solid foundation for treating refractory thrombocytopenia patients who require platelet transfusions. HLA-A, -B epitope phenotype data were collected from a total of 565 unrelated healthy populations in the Jining area. According to the data, a platelet donor database was created and established with known HLA epitope phenotypes. The database donors' HLA-A, -B epitopes were tested separately by sequence-specific primer (PCR-SSP). According to the distribution frequency of HLA-A, -B epitope phenotypes (n = 565), the following results were obtained: 73 HLA-A, -B phenotypes, including 55 with a frequency > 0.003. The sum of frequencies was 96.81%. With a database size of 565 donors, an approximate 84.20% patient HLA-A, -B cross-reactive group (CREG) match coverage was achieved. When it was necessary for identical ABO-type platelet transfusion, the corresponding database modifications were made according to the distribution frequency of the ABO blood group of the population.This study provides the concept for a platelet epitope matching database. It establishes a donor database of known platelet HLA epitopes in the Jining population, which lays a solid foundation for the safe treatment of platelet transfusion in this area.