RHD haplotype analysis of a D negative cohort

This study aimed to explore the distribution of the variant RHD genotypes in Jiangsu, China. A total of 108541 blood donor samples and 17 family members were tested from 2020 to 2021. A total of 121 RHD variants were found in 414 D negative samples and their families, including seven weak D, four partial, and three Del alleles. RHD*15 and RHD*DVI.3 were the prevalent D variant alleles in Jiangsu Province. Heterozygous alleles were identified (c.730G>C, c.1227G>A), and a case of unique intronic mutation was detected (c.802-41_802-38delCTCT). This study suggests that the frequency of RHD variants is crucial for establishing specific RHD genotyping strategies and can be applied in larger-scale routine tests for blood donors.