Application of new enhanced medium in indirect antiglobulin test by gel method

The objective of this study was to explore the efficacy of a new enhanced medium in indirect antiglobulin test by optimized gel method. Anti-IgG+ C3d and anti-IgG antiglobulin gel cards, and samples from three blood group systems: Rh, Kidd and MNSs were used. The new enhanced medium was incubated at 37°C for 5 minutes, low ionic strength solution (LISS) for 15 minutes, saline solution for 30 minutes, respectively. The sensitivity and specificity of blood group antibodies were then compared. The results showed that the specificity and sensitivity of the new enhanced medium incubated for 5 minutes were consistent with those of LISS and saline solution for 15 minutes and 30 minutes, respectively. The results suggest that the new enhanced medium be able to accelerate the binding of erythrocyte antibodies with antigens, reduce incubation time and optimize the detection process of indirect antiglobulin test by gel method.