Haochun Chang, Hsin-Wan Chen, Chen Cao, Xiaojie Zhu, Zhenzhen Zhou, Yu-Shiang Lin. Polymorphism of SLC14A1 encoding human erythrocytic Kidd antigens in the Chinese population[J]. Blood&Genomics, 2017, 1(4): 43-46. DOI: 10.46701/APJBG.2017042017056
Citation: Haochun Chang, Hsin-Wan Chen, Chen Cao, Xiaojie Zhu, Zhenzhen Zhou, Yu-Shiang Lin. Polymorphism of SLC14A1 encoding human erythrocytic Kidd antigens in the Chinese population[J]. Blood&Genomics, 2017, 1(4): 43-46. DOI: 10.46701/APJBG.2017042017056

Polymorphism of SLC14A1 encoding human erythrocytic Kidd antigens in the Chinese population

More Information
  • Available Online: 2021-06-29
  • The SLC14A1 gene, which encodes the important Kidd blood group antigens, has not been systematically analyzed at the molecular level in Chinese individuals. In this study, SLC14A1 genetic polymorphism was examined in Chinese individuals with Jk(a+b-), Jk(a+b+), and Jk(a-b+) expression. The Kidd phenotype was determined for 146 specimens using monoclonal anti-Jka and -Jkb antibodies. From these, 87 specimens were Jk(a-b+), 21 were Jk(a+b-), and 38 were Jk(a+b+). According to the Kidd phenotype results, 20 specimens were randomly selected from each group, i.e., Jk(a-b+), Jk(a+b-), and Jk(a+b+), for the molecular analyses of exons 3 to 11 of the SLC14A1 gene. Novel alleles were detected in the SLC14A1 gene, including IVS3-106A, IVS3-99A, exon3 130G, IVS4-299G, IVS4-293G, IVS4+211C, IVS4 +230C, exon6 499A, exon6 588A, IVS7-68T, IVS9+244G, and IVS10-153T, indicating that the locus harbored significant polymorphism. We also showed that IVS4-299, IVS7-68, and IVS10-153 were novel SNPs absolutely associated with exon 8 nt. 838. The minor allele frequencies were all greater than 10% and all SNPs in the Chinese population showed Vel antigen expression on RBC membranes. We identified 12 SNPs in the SLC14A1 gene in the Chinese population, IVS3-106A, IVS3-99A, exon3 130G, IVS4-299G, IVS4-293G, IVS4+211C, IVS4 +230C, exon6 499A, exon6 588A, IVS7-68T, IVS9+244G, and IVS10-153T. Our results also indicated that three novel SNPs produced Jka and Jkb antigens in Chinese individuals.
  • Related Articles

    [1]Tingting Yang, Zhenqing Feng. Overview of the diversity of erythrocyte blood group antigens[J]. Blood&Genomics, 2020, 4(2): 115-122. DOI: 10.46701/BG.2020022020123
    [2]Qing Liu, Jiayan Ling, Qilong Song, Xiaodong Li, Chizhi Zhang, Mingzhong Xiao, Jie Huang, Shuxia Han, Ling Liu, Xiaoming Peng, Jie Tan, Yu Wang, Li Ouyang, Youyun Zhao, Jianhua Wu. Association between polymorphism of the cyclin E1 gene and susceptibility to hepatocellular carcinoma in Chinese Han population of Hubei[J]. Blood&Genomics, 2019, 3(2): 125-131. DOI: 10.46701/APJBG.2019022019118
    [3]Dongmei Li, Dongjing Liu, Yi Zha, Tao Wu, Yan Qiu. The polymorphisms of HLA class Ⅰ and Ⅱalleles were not associated with severe acute respiratory syndrome among recovered patients in Beijing: a case-control study[J]. Blood&Genomics, 2019, 3(1): 19-25. DOI: 10.46701/APJBG.2019012018140
    [4]Guadalupe E. Estrada-Chávez, Roberto Estrada, Guadalupe Chavez, María-Elisa Vega Memije, Roberto Arenas Guzmán, Maricela García-Lechuga, Julio Granados, Lucia Rangel-Gamboa. HLA class Ⅱ alleles in human sporotrichosis in Mexican Amerindians[J]. Blood&Genomics, 2018, 2(3): 183-190. DOI: 10.46701/APJBG.2018032018119
    [5]Yanjun Jia, Wei Li, Lijun Wang, Na Liu, Dongmei Wang, Dongmei Li, Yuanyuan Jing, Jie Wang, Zhongmei Wang, Xiaoyan Shan. Allele and haplotype frequencies of human leukocyte antigen-A, -B, -C,-DRB1, and -DQB1 in Chinese patients with hematological diseases[J]. Blood&Genomics, 2018, 2(1): 27-37. DOI: 10.46701/APJBG.2018012017064
    [6]Huan Wang, Xiaojun Tang, Xin Xu, Chu Chu, Siping Xiong, Feng Zheng, Qisong Peng. The establishment of human anti-TROP2 antibody IgG and its inhibition function on pancreatic cancer cell proliferation[J]. Blood&Genomics, 2017, 1(4): 65-71. DOI: 10.46701/APJBG.2017042017045
    [7]Na Ma, Zhiyuan Xu, Yushiang Lin, Haochun Chang, Xiaofei Li, Daowang Fan, Yan Qiu, Tianhong Miao. Mini-review to analyse the phenotype, genotype, and alloantibody titre against Diego antigens in the Chinese population[J]. Blood&Genomics, 2017, 1(4): 55-60. DOI: 10.46701/APJBG.2017042017052
    [8]Fang-Yeh Chu, Lung-Chih Yu, Chih-Chun Chang, Marie Lin. Molecular genetic analysis of the Kidd blood group system in a Taiwanese family with Jknull phenotype[J]. Blood&Genomics, 2017, 1(4): 29-35. DOI: 10.46701/APJBG.2017042017050
    [9]Yuping Chen, Tongmao Zhao. Application of a multiplex PCR genotyping assay of 31 red blood cell antigens for establishment of a panel of reference DNA in China[J]. Blood&Genomics, 2017, 1(4): 13-16. DOI: 10.46701/APJBG.2017042017055
    [10]Pu Xu, Yan Li, Su Zhou, Ziqi He, You Yang, Hua Yu. Human RBC alloimmunization evaluation after exposure to foreign E antigen of the rhesus system in transfusion[J]. Blood&Genomics, 2017, 1(1): 37-42. DOI: 10.46701/APJBG.20170116009

Catalog

    Article Metrics

    Article views (1626) PDF downloads (1066) Cited by()
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return